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In mycobacteriology, the switch from the phenotypic approach to the genotypic one has not been as quick as in other microbiological fields.

A large popularity was in fact gained in the 1990s by the chemotaxonomic methods based on the analysis of cell wall lipids.

Without claiming to be authoritative or comprehensive, this note aims to provide a few simple directions suitable to steer the choice of the best possible identification.

The accurate identification of bacteria is becoming more and more problematic, mainly because of the steady increase of the number of species.

Such technique, limited at first to highly specialized laboratories, has become in recent years very popular mainly due to the robustness of the chemistry (based on the Big Dye Terminator technology) and to the excellent performance of modern automatic instrumentations.